9,141 research outputs found
The Second Triennial Systematic Literature Review of European Nursing Research: Impact on Patient Outcomes and Implications for Evidence-Based Practice
Get PDFWritten on behalf of the European Academy of Nursing Science REFLECTION review group: José Amendoeira, Polytechnic Institute of Santarem, Santarem, Portugal; (…) p.9European research in nursing has been criticized as overwhelmingly descriptive, wasteful and with little relevance to clinical practice. This second triennial review follows our previous review of articles published in 2010, to determine whether the situation has changed.info:eu-repo/semantics/publishedVersio
Sexual Preference as a Suspect (Religious) Classification: An Alternative Perspective on the Unconstitutionality of Anti-Lesbian/Gay Initiatives
Get PDFLiberalism, Public Morality, and Constitutional Law: Prolegomenon to a Theory of the Constitutional Right to Privacy
Get PDFHuman Rights and Criminal Punishment (reviewing \u3ci\u3ePunishment, Danger and Stigma: The Morality of Criminal Justice\u3c/i\u3e by Nigel Walker)
Get PDFCommercial Sex and the Rights of the Person: A Moral Argument for the Decriminalization of Prostitution
Get PDFEqual Opportunity and School Financing: Towards a Moral Theory of Constitutional Adjudication
Get PDFSpatial Regulation of Exocytic Site and Vesicle Mobilization by the Actin Cytoskeleton
Get PDFNumerous studies indicate a role for the actin cytoskeleton in secretion. Here, we have used evanescent wave and widefield fluorescence microscopy to study the involvement of the actin cytoskeleton in secretion from PC12 cells. Secretion was assayed as loss of ANF-EmGFP in widefield mode. Under control conditions, depolarization induced secretion showed two phases: an initial rapid rate of loss of vesicular cargo (tau = 1.4 s), followed by a slower, sustained drop in fluorescence (tau = 34.1 s). Pretreatment with Latrunculin A changed the kinetics to a single exponential, slightly faster than the fast component of control cells (1.2 s). Evanescent wave microscopy allowed us to examine this at the level of individual events, and revealed equivalent changes in the rates of vesicular arrival at the plasma membrane immediately following and during the sustained phase of release. Co-transfection of mCherry labeled β-actin and ANF-EmGFP demonstrated that sites of exocytosis had an inverse relationship with sites of actin enrichment. Disruption of visualized actin at the membrane resulted in the loss of specificity of exocytic site
Book Reviews
Get PDFBook Reviews of: Carol Tavris, Anger: The Misunderstood Emotion (Simon & Schuster, 1982) John Fowles, A Maggot (Little-Brown, 1985) James Q. Wilson and Richard J. Herrnstein, Crime and Human Nature (Simon & Schuster, 1985
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